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Bees in a tree cavity

Atmore, AL(Zone 8b)

Last summer there were lots of bees coming from this tree cavity (hole), and they were swarming up to 20ft from the tree. I read a report a few days ago where africanized "killer" bees were caught near New Orleans. That's not very from from me so I don't want to take any chances, this tree is near my walking trail through the woods. Is there any way that I can make sure they don't colonize this hole again?

Thumbnail by escambiaguy
Kalispell, MT(Zone 4b)

Fill the hole with wax. I doubt the queen would search again and remove such a barrier.

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